With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. For more sample throughput, the system canThe optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). 94 g calcium chloride dihydrate (both. All strains were tested three times and the results averaged. Readings were made in a microplate spectrophotometer (SpectraMAX 340, Molecular Devices, Sunnyvale, California, USA) with wavelength set to 450 nm and correction set. EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen. The SpectraMax GEMINI EM cannot be operated without the computer and SoftMax Pro software. GE Healthcare spectramax 340 pc plate reader Spectramax 340 Pc Plate Reader, supplied by GE Healthcare, used in various techniques. Assay setup for a 384-well low-volume plate. SpectraMax® Microplate Readers and SoftMax® Pro Software. FXII or PK activation by FXIa was tested as follows: FXII or PK (200nM) was incubated with or without FXIa (25nM) in TBS, 0. ELISAs were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 0 2. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. 4; 100 m M NaCl, 0. Please see photos. Buffer (100 mmol L –1acetate buffer, pH 5. Donor filter: 620 nm. 00. The SPECTRAmax 340PC can mea-The extracted stain was then transferred to a 96-well plate, and the absorbance at 562 nm was measured using a SpectraMax 340 plate reader/spectrophotometer(Molecular Devices Corp. The optical system of the SpectraMax 340PC Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). SpectraMax® M2/M2e user guide a multi-detection microplate reader with two-mode cuvette port SpectraMax M2e user guide cover 1 1 4/21/06 9:53:28 AM. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. Figure 1. Filipin StainingSpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. 315 can't find zero order. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. 5. Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). Details. Molecular Device SpectraMax M3 Plate Reader - Certified with Warranty. Acceptor filter: 665 nm. 2 Chemical Reagents 1. Page: 1/6. Donor: 616/10 nm. Revvity. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. The cell concentration was measured by a spectrometer (SpectraMax 340, Molecular Devices, USA) with an optical density at 600 nm (OD 600). The reaction mixture was incubated for 5 min. SpectraMax 190 reads 96-well and strip-well plates. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San. The absorbances were transformed to velocity of substrate consumption (nmol/min). #PLUS 384) MethodsSpectraMax 340 PC, Molecular Devices, USA was used as a 96-well microplate reader to test bioactivities. 5% (w/v) sodium dodecyl sulfate-polyacrylamide gels, and transferred to immobilon polyvinylidenedifluoride membranes (Millipore, Bedford, USA). 00 USD. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. Cell viability was determined using the blue formazan assay in which colorless 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is metabolized to a blue product by mitochondrial dehydrogenases. TDP-43 stress granular cells were treated with 70% ice-cold. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. The relative LDH release was determined as the percentage ratio of the optical densities in the media (containing CNMs at each. 0 to 4. Absorbance was recorded at 540 nm using SpectraMAX 340 reader. 2. NO release assayThe SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. 4) was followed according to Vanacker et al. 5. Triton X-100 (see Note 2). below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Each experiment was performed in duplicate and repeated at. 340 nm / 70 nm. P. Service Contracts, Preventative Maintenance and Performance. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. 9000-0756. Page 1 ® SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0049 Rev. The SpectraMax® iD3 Multi Mode Microplate . Supplier: Molecular Devices. 003 OD Stray light ≤ 0. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. Anti-proteolytic activity. Dissolve 13. Plates were washed with 1× KP solution and blocked with. The OD for each serum is adjusted by subtracting either the OD for spontaneous mediator release or antigen-specific mediator release. Amino acid analysis was performed on a Pico-Tag amino acid analyzer (Wat er System) as. Setup recommendations for SpectraMax. 190-1000 nm. ATI/240, 250. ScanLater Western Blot Detection Cartridge. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). The relative cell viability was determined as the percentage ratio of the optical densities in the medium (containing the nanoprobes at each concentration) to that of the fresh control medium. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. TGFα release was calculated as described in the Results. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. For more sample throughput, the system can The optical absorption was measured at 450 nm for BUN and 490 nm for creatinine with a spectrometer (SpectraMax 340). The SPECTRAmax 340PC can measure. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. SpectraMax 340 Absorbance Microplate Reader, Molecular Devices (CAT#: STEM-SPO-0021-ZJF) Labsystems Fluoroskan II Fluorescent Microplate Reader, Excitation Wavelength Range 320 – 700 nm, Labsystems (CAT#: STEM-SPO-0006-LGZ) BioTek MQX200 Microplate Reader Spectrophotometer, 24VDC, BioTek Instruments, Inc (CAT#: STEM. Homeostatic model assessment for insulin. Samples containing 25 μg of protein were separated on 12. Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. The absorbances were being measured at 515 nm. MCE has not independently confirmed the accuracy of these methods. Data were expressed as the mean percent viable cell vs. At that point halfway up the maximum transmittance value, the total width (nm) of the transmitted band is. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. 8–250 nM) was measured as follows: the prothrombinase reaction proceeded for 3 min and was stopped by the addition of 10 mM EDTA. 2. The ability of PTK787/ZK 222584 to inhibit angiogenesis was tested in an in vitro model of capillary sprout formation (64) . The loading equaled the mass of BSA per mass of particles. Read one sample or up to 384. Optical density was determined at 490 nm (background values measured at 690 nm were subtracted) using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). The extreme flexibility and high sensitivity of the SpectraMax GEMINI EM make it appropriate for applications within the fields of bio-A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 5. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. 313 reference gain check fail. Acceptor: 665/10 nm. Integration delay. Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of. Nitrocefin hydrolysis was monitored at 482 nm on a SpectraMax 340 UV-visible plate reader for high-throughput assays and an HP8453 UV-visible spectrophotometer for the low-throughput assays. The relative cell viability was determined as the percentage of the absorbance value in the cells to the absorbance value of a control group. measurements were performed on a SPECTRAmax-340 plate reader (Molecular Devices Inc. TGFα release was calculated as described in the Results. Near bottom. Operators can conveniently select up to 6 wavelengths at a time for. The absorbance was measured at 550 nm with a Spectramax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). SpectraMax Multi-Mode Microplate Readers. Here, we show how the SpectraMax® i3x and SpectraMax® iD5 Multi-Mode Microplate Readers are used to perform robust, no-wash saturation binding assays using HTRF. Incubate 30 min at room temperature, and then read microplates at 405 nm using SpectraMax 340 PC plate reader (Molecular Devices) (see Note 8). Plates coated with no antigen, canine CD19, streptavidin alone, and streptavidin with irrelevant human avitag-CD3ε (Acro Biosystems, Newark, DE) were used as negative controls. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. SpectraMAX 340. TDP-43 stress granular cells were treated with 70% ice-cold. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). 6. 4) was followed according to Vanacker et al. Data were expressed as mean percentage of viable cells. Statistical analysis. 2. Measurements of OD at 540 nm were then performed in a microtiter plate reader (SpectraMax 340 microplate reader; Molecular Devices Corp. ): 8. , San Jose, CA) using Softmax Pro 7 software. 00 USD. The assays contained 0. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). 30. Following incubation, 100 μl of solution was transferred to new wells and the color change in the solution was measured with a microtiter plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. 1% PEG 8000 at 37°C. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature. SpectraMax Injector Cartridge with SmartInject™ Technology. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). Absorbance values were read at 570 nm on an automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA). Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. . Cell Culture AO391 (derived from human aorta) and CD314 (derived from human carotid artery) cell lines were a generous gift from Wendy Bauer (Tufts Molecular Cardiology Research Institute) and were grown in DMEM (CellGro) supplemented with 10% FBS. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. The plates were incubated for 4 additional hours at 37°C. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature. Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. 4. Software (SoftMax Pro ® software or comparable plate reader software). TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross. Enzyme activities at lower concentrations were assayed by using a LS50B fluorometric. Molecular Devices Spectramax 340 Plate Reader Repair. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Includes: Computer Loaded with Softmax Pro 5. The levels of rheumatoid factor (RF) antibodies were measured at the Clinical Immunology Laboratory of the Sahlgrenska University Hospital or Leiden Clinical Immunology Laboratory. VersaMax reads 96-well and strip-well plates. using SpectraMax i3 (Molecular devices, San Jose, USA). Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. SpectraMax 340 from Molecular Devices). Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. . The. Subsequent replacements require only the lamp (N-34-LB) be replaced. Call 1-800-745-2710. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. Includes: SpectraMax 340PC384. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid- plates can be used in the SPECTRAmax PLUS 384. Plasma glucose levels were measured using FreeStyle Lite (Abbott Diabetes Care Ltd. Alves, Amelia K. Amino acid analysis was performed on a Pico-Tag amino acid analyzer (Wat er System) as. These lab workhorses are used in drug discovery, research, bioassay validation, and. 1% Chaps, 10 m M dithiothreitol, and 10% sucrose. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. A standard curve was constructed by sequential dilution of a TNFα standard from 2000-15 pg/mL. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). 30. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. These isotypes cause antigen cross-linking by multimeric binding and self. Table 1. Thin layer chromatography (TLC) was. a SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). It measures the optical density (OD) of samples in both 96- and 384-well microplates at a selected wavelength for a single point in time (endpoint), over a specified period of time. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. Quick View. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Temperature Range: Ambient + 4 °C up to 45 °C. Stat Fax 4700 Microstrip Reader. The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). Microplate Spectrophotometer Operator’s Manual. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 304 signal level saturation. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Fibrin clots were formed as described above with some modifications 46. TDP-43 stress granular cells were treated with 70% ice-cold. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. , Sunnyvale, CA). 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the enhanced TRF detection module for the SpectraMax iD5 Multi-Mode Microplate ReaderThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. All absorbance measurements were taken on a SpectraMax 340 PC reader equipped with SoFTMax Pro software. Liquid handling for random screening was carried out with a Biomek FX from Beckman Coulter (Fullerton, CA, USA) and HYTRA. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Reagena Poc Puumala® was used for detection of IgM antibody against PUUV in NE patient sera at the Department of Clinical Virology,. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. , Sunnyvale, CA). Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. 340/70 nm. The optical density at 405 nm (OD 405) of the plates was measured with a microplate reader (SpectraMax 340 PC384, Molecular Devices) before and after a 1-h incubation at room temperature. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. , Sunnyvale, CA). The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The ratio of red to green fluorescence intensity was calculated. Accurate quantitation The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. SpectraMax Plus 384 measuring instruments pdf manual download. This light offers spectrum variability, wireless control, and photosensors to automatically balance output with natural light. 1 mM MnCl2 and 25 mM CHES buffer, pH 9. Molecular Devices SpectraMax M5e Microplate Reader. Upgradeable modules are also available including western blot, cell imaging, and fast. In order to detect the Fc-tagged fusion proteins, goat anti-human IgG (Fc Fragment specific; Jackson ImmunoResearch Laboratories Inc, West Grove, PA, USA; Code Number 109-006-098;. See More Microplate Reader / Microtiter Plate Reader. Irene Tieleman, Ido PenandSimon VerhulstSubsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 5 hours, and is measured in a 96-well plate spectrophotometer at 405 nm (SpectraMax 340; Molecular Devices LLC, Sunnyvale, CA, USA). Amino Acid Analysis. 1 in stock! Molecular Devices SpectraMax M2 Multilabel Microplate Reader. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. A microplate reader asThe absorbance was measured at 425 nm and read every 10 min using a Spectramax 340 plate reader (Molecular Devices ®, USA). Intra- and inter-assay coefficients of variation were lower than 8. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. or microplate reader (e. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027 EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen AlphaScreen and AlphaLisa detection using 1 W 680 nm EX laser diode and a 570 nm (100) EM filter Pick best speed, sensitivity, and price for your needs Table 1. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the visible range. Unit powers. After the addition of sPLA2 (20 μ g), the reaction mixture was incubated for 40 min at 37°C, and the absorbance was read at 10 min intervals. 2. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). 1. Binding of capture antibodies to polystyrene beads and biotinylation. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. Acceptor: 665nm. Details. Created Date: 20051213090753Z measured using a SpectraMax 340 multiwell plate. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. Incubation with TMB substrate was done for 18 min. 5. Call 1-800-745-2710. Isolation of RNA and RT-PCR. 7 macrophages were obtained from ATCC (Manassas, VA). After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). Growth was monitored at 30°C every 6 min for 8 to 12 h at 660 nm using a multiscan photometer (spectraMax 340; Molecular Devices). SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer to the Obsolete Microplate Reader Models article for additional information. 0 nm increments Wavelength Bandwidth: 2 nm Wavelength Accuracy: < ± 1. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Microplate Readers. chen@case. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Wallac 1450 Microbeta TRILUX Liquid Scintillation & Luminescence Counter $1,000. 312 gain calibration failed. Each SpectraMax® 340 PC 384 Microplate Reader has an. Assay buffer was 50 m M Hepes, pH 7. Data were expressed as the mean LPS-treated cells (100%) vs. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). Clotting times (mean ± SD) for rSimukunin and rSV. ” SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Western blot. The results are expressed as the percentage inhibition of BrdU incorporation compared to the untreated control. The antibodies originally defining this molecule by inducing apoptosis, anti-Fas and anti-APO-1 , were of the IgM or IgG3 subtype, respectively. 30. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise. 0, MSD) or Spectramax 340 (Molecular Devices). The VersaMax and SpectraMax 190 read 96-well microplates. Call 1-800-745-2710. 3T3 cells were cultured in DMEM supplemented with 10% FCS and 1% antibiotics (10,000 units/ml penicillin and 10,000 μg/ml streptomycin in 0. Molecular Devices Corporation 1311. 0 3. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Details. 3. The plate was covered and incubated for one hour at room temperature. (1988) and recorded at 412 nm. Italic and boldface type are used for emphasis. 0 OD. 1% N-1-naphtylethylenediamine dihydrochloride, 2. The cell proliferation on different Ti discs was assessed by measuring the absorbance at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Devices, Sunnyvale, CA). When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. , Sunnyvale, CA). Binding affinity for denosumab. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Photometric Range: 0. Molecular Devices. One plate carrier adapter is provided with the instrument. Donor filter: 616/10 nm. 5 mL sample or standard solution was well mixed with 0. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. Proteolytic activity was determined using the synthetic chromogenic substrate Nα-benzoyl-DL-arginine p-nitroanilide (DL-BApNA), in 96-well plates, according to Ponce-Soto et al. 190–850 nm <± 1. Wavelength range: 340-850 nm. 306 plate air cal. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. HTRF-compatible SpectraMax Multi-Mode Readers. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. SpectraMax QuickDrop Micro-Volume Spectrophotometer. Call 1-800-745-2710. used in the SPECTRAmax PLUS. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. The values of IC 50 represent concentrations of compounds toA microplate reader (SpectraMax M5; Molecular Devices, Sunnyvale, CA) was used to measure the absorbance at 340 nm for the collected BPW (6) Stabilization protects islet integrity during respirometry in the Oroboros Oxygraph-2K analyzer Islets December 31, 2022 Justin J. The absorbance at 450 nm was determined using a microplate reader (spectraMAX 340). Molecular Devices SpectraMax Gemini XS Microplate Reader. Cell proliferation was measured using the MTT method. Immunofluorescence and Aggregation Analysis. Bertin Precellys Evolution Touch Homogenizer. We also offer depot Repair Service from our base of operations in Southern California (San Diego). 001/min at 425 nm. Despite the relevance of adaptive immunity against equine pathogens antigen-specific T cell responses of horses are not well characterized and the lack of insight into T cell responses hampers the understanding of the pathogeneses of important diseases. All assays were done in triplicate with readings at 60-s intervals using a SpectraMax 340 multiwell plate reader (Molecular Devices, San Jose, CA, United States). This is equivalent to having 511 built-in filters,Results: A total of 64% of the subjects reported improved quality of sleep during treatment with naloxone at a dose of 40 ng per 24 hours as compared with 9% with sham treatment (P=0. On-site Repair Service available nationwide for Molecular Devices Spectramax 340 Plate Reader's. Since the formation of NADPH is stoichiometrically related to the oxidation of glucose-6-phosphate, measurements for glucose + fructose can be determined by the absorbance of NADPH at 340 nm. 1. , Sunnyvale, CA). Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. AoSMCs were seeded into 6-well culture plates. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. SpectraMax ® Plus 384 optics. A. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. On 1, 7, 14, and 21 days, the cells were washed with DPBS twice and incubated with CCK-8 solution for 2 h in the dark at 37 °C. chen@case. 5 mL sample or standard solution was well mixed with 0. The mixture changed to a red color by a reaction of LDH that was measured by a plate reader (SpectraMax 340 PC; Molecular Devices, San Jose, CA, USA) at a visible wavelength of. SpectraMax Multi-Mode Microplate Readers. As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. 7. SoftMax Pro. FACS analysis. Molecular Device M5e. The. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. Fig. Samples were analyzed in triplicates. 0. 3 mm. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. StakMax Microplate Handling Stacker. Our absorbance plate readers feature our PathCheck Sensor technology and. Awareness Technology, Inc. Plasminogen was added to the fibrinogen before addition of thrombin and either tissue plasminogen activator (tPA) or urokinase plasminogen activator in the. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance plate reader (Molecular Devices, Union City, CA). Then, the absorbance value at 450 nm was determined using a microplate reader (SpectraMAX 340; Molecular Devices, Sunnyvale, CA, United States). Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Figure 1. Driessen*,MaaikeA. The reduction of HED in the presence of NADPH and GR was recorded at 340 nm. ATI Note: #1: The N-34-MLA is a replacement assembly which replaces an OEM lamp. Novak, Nathan J. The concentration of NO in the cells was determined by measuring the amount of nitrite, a relatively stable oxidation product of.